Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus.
Identifieur interne : 000386 ( Main/Exploration ); précédent : 000385; suivant : 000387Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus.
Auteurs : El Hadj Driche [Algérie] ; Nasserdine Sabaou [Algérie] ; Christian Bijani [France] ; Abdelghani Zitouni [Algérie] ; Frédéric Pont [France] ; Florence Mathieu [France] ; Boubekeur Badji [Algérie]Source :
- World journal of microbiology & biotechnology [ 1573-0972 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Afrique du Nord (MeSH), Analyse de séquence d'ADN (méthodes), Furanes (pharmacologie), Microbiologie du sol (MeSH), Phylogenèse (MeSH), Résistance à la vancomycine (effets des médicaments et des substances chimiques), Staphylococcus aureus résistant à la méticilline (effets des médicaments et des substances chimiques), Streptomyces (classification), Streptomyces (isolement et purification), Streptomyces (métabolisme), Techniques de typage bactérien (MeSH), Tests de sensibilité microbienne (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Résistance à la vancomycine, Staphylococcus aureus résistant à la méticilline.
- génétique : ARN ribosomique 16S, Streptomyces.
- isolement et purification : Streptomyces.
- métabolisme : Streptomyces.
- méthodes : Analyse de séquence d'ADN.
- pharmacologie : Furanes.
- Afrique du Nord, Microbiologie du sol, Phylogenèse, Techniques de typage bactérien, Tests de sensibilité microbienne.
English descriptors
- KwdEn :
- Africa, Northern (MeSH), Bacterial Typing Techniques (MeSH), Furans (pharmacology), Methicillin-Resistant Staphylococcus aureus (drug effects), Microbial Sensitivity Tests (MeSH), Phylogeny (MeSH), RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA (methods), Soil Microbiology (MeSH), Streptomyces (classification), Streptomyces (isolation & purification), Streptomyces (metabolism), Vancomycin Resistance (drug effects).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical , pharmacology : Furans.
- classification : Streptomyces.
- drug effects : Methicillin-Resistant Staphylococcus aureus, Vancomycin Resistance.
- isolation & purification : Streptomyces.
- metabolism : Streptomyces.
- methods : Sequence Analysis, DNA.
- Africa, Northern, Bacterial Typing Techniques, Microbial Sensitivity Tests, Phylogeny, Soil Microbiology.
Abstract
A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368T with 99.6% of similarity. However, the comparison of the morphological and the physiological characteristics of the strain with those of the nearest species showed significant differences. The strain AT37 secreted the antibiotic optimally during mid-stationary phase of growth. One active compound (AT37-1) was extracted from the culture broth with dichloromethane, separated on silica gel plates and purified by HPLC. Based on spectroscopic analysis of UV-Visible, infrared, and 1H and 13C NMR spectra and spectrometric analysis, the chemical structure of the compound AT37-1 was identified as 5-[(5E,7E,11E)-2,10-dihydroxy-9,11-dimethyl-5,7,11-tridecatrien-1-yl]-2-hydroxy-2-(1-hydroxyethyl)-4-methyl-3(2H)-furanone. Minimum inhibitory concentrations and minimum biofilm inhibitory concentration (MBIC50) of this compound showed significant activity against multidrug-resistant S. aureus with 15-30 and 10-15 μg/mL, respectively.
DOI: 10.1007/s11274-017-2265-y
PubMed: 28466299
Affiliations:
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Le document en format XML
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AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus.</title><author><name sortKey="Driche, El Hadj" sort="Driche, El Hadj" uniqKey="Driche E" first="El Hadj" last="Driche">El Hadj Driche</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger</wicri:regionArea><placeName><settlement type="city">Alger</settlement><region nuts="2">Wilaya d'Alger</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université Hassiba Benbouali de Chlef, Hay Salem, Chlef, 02000, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Département 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wicri:level="3"><nlm:affiliation>Laboratoire de chimie de coordination (LCC), CNRS, Université de Toulouse, UPS, INPT, LCC, 205, route de Narbonne, 31077, Toulouse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de chimie de coordination (LCC), CNRS, Université de Toulouse, UPS, INPT, LCC, 205, route de Narbonne, 31077, Toulouse</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Zitouni, Abdelghani" sort="Zitouni, Abdelghani" uniqKey="Zitouni A" first="Abdelghani" last="Zitouni">Abdelghani Zitouni</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger</wicri:regionArea><placeName><settlement type="city">Alger</settlement><region nuts="2">Wilaya d'Alger</region></placeName></affiliation></author><author><name sortKey="Pont, Frederic" sort="Pont, Frederic" uniqKey="Pont F" first="Frédéric" last="Pont">Frédéric Pont</name><affiliation wicri:level="3"><nlm:affiliation>Proteomics Group, INSERM UMR1037, Centre de Recherches en Cancérologie de Toulouse (CRCT), Toulouse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Proteomics Group, INSERM UMR1037, Centre de Recherches en Cancérologie de Toulouse (CRCT), Toulouse</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Midi-Pyrénées</region><settlement type="city">Toulouse</settlement></placeName></affiliation></author><author><name sortKey="Mathieu, 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xml:lang="fr">Algérie</country><wicri:regionArea>Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger</wicri:regionArea><placeName><settlement type="city">Alger</settlement><region nuts="2">Wilaya d'Alger</region></placeName></affiliation></author></analytic><series><title level="j">World journal of microbiology & biotechnology</title><idno type="eISSN">1573-0972</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Africa, Northern (MeSH)</term><term>Bacterial Typing Techniques (MeSH)</term><term>Furans (pharmacology)</term><term>Methicillin-Resistant Staphylococcus aureus (drug effects)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA (methods)</term><term>Soil Microbiology (MeSH)</term><term>Streptomyces (classification)</term><term>Streptomyces (isolation & purification)</term><term>Streptomyces (metabolism)</term><term>Vancomycin Resistance (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (génétique)</term><term>Afrique du Nord (MeSH)</term><term>Analyse de séquence d'ADN (méthodes)</term><term>Furanes (pharmacologie)</term><term>Microbiologie du sol (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Résistance à la vancomycine (effets des médicaments et des substances chimiques)</term><term>Staphylococcus aureus résistant à la méticilline (effets des médicaments et des substances chimiques)</term><term>Streptomyces (classification)</term><term>Streptomyces (isolement et purification)</term><term>Streptomyces (métabolisme)</term><term>Techniques de typage bactérien (MeSH)</term><term>Tests de sensibilité microbienne (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Furans</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Methicillin-Resistant Staphylococcus aureus</term><term>Vancomycin Resistance</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Résistance à la vancomycine</term><term>Staphylococcus aureus résistant à la méticilline</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Streptomyces</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de séquence d'ADN</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Furanes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Africa, Northern</term><term>Bacterial Typing Techniques</term><term>Microbial Sensitivity Tests</term><term>Phylogeny</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Afrique du Nord</term><term>Microbiologie du sol</term><term>Phylogenèse</term><term>Techniques de typage bactérien</term><term>Tests de sensibilité microbienne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368<sup>T</sup> with 99.6% of similarity. However, the comparison of the morphological and the physiological characteristics of the strain with those of the nearest species showed significant differences. The strain AT37 secreted the antibiotic optimally during mid-stationary phase of growth. One active compound (AT37-1) was extracted from the culture broth with dichloromethane, separated on silica gel plates and purified by HPLC. Based on spectroscopic analysis of UV-Visible, infrared, and <sup>1</sup>H and <sup>13</sup>C NMR spectra and spectrometric analysis, the chemical structure of the compound AT37-1 was identified as 5-[(5E,7E,11E)-2,10-dihydroxy-9,11-dimethyl-5,7,11-tridecatrien-1-yl]-2-hydroxy-2-(1-hydroxyethyl)-4-methyl-3(2H)-furanone. Minimum inhibitory concentrations and minimum biofilm inhibitory concentration (MBIC<sub>50</sub>) of this compound showed significant activity against multidrug-resistant S. aureus with 15-30 and 10-15 μg/mL, respectively.</div></front></TEI><affiliations><list><country><li>Algérie</li><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li><li>Wilaya d'Alger</li></region><settlement><li>Alger</li><li>Toulouse</li></settlement></list><tree><country name="Algérie"><region name="Wilaya d'Alger"><name sortKey="Driche, El Hadj" sort="Driche, El Hadj" uniqKey="Driche E" first="El Hadj" last="Driche">El Hadj Driche</name></region><name sortKey="Badji, Boubekeur" sort="Badji, Boubekeur" uniqKey="Badji B" first="Boubekeur" last="Badji">Boubekeur Badji</name><name sortKey="Driche, El Hadj" sort="Driche, El Hadj" uniqKey="Driche E" first="El Hadj" last="Driche">El Hadj Driche</name><name sortKey="Sabaou, Nasserdine" sort="Sabaou, Nasserdine" uniqKey="Sabaou N" first="Nasserdine" last="Sabaou">Nasserdine Sabaou</name><name sortKey="Zitouni, Abdelghani" sort="Zitouni, Abdelghani" uniqKey="Zitouni A" first="Abdelghani" last="Zitouni">Abdelghani Zitouni</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Bijani, Christian" sort="Bijani, Christian" uniqKey="Bijani C" first="Christian" last="Bijani">Christian Bijani</name></region><name sortKey="Mathieu, Florence" sort="Mathieu, Florence" uniqKey="Mathieu F" first="Florence" last="Mathieu">Florence Mathieu</name><name sortKey="Pont, Frederic" sort="Pont, Frederic" uniqKey="Pont F" first="Frédéric" last="Pont">Frédéric Pont</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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